Year: 2020
Type: article
Abstract: Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter ( TCSn::GFP ) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses.
Source: Nature Communications
Authors: Ioanna Antoniadi, Ondřej Novák, Zuzana Gelová, Alexander Johnson, Ondřej Plíhal, Radim Simerský, Václav Mik, Thomas Vain, Eduardo Mateo-Bonmatí, Michal Karady, Markéta Pernisová, Lenka Plačková, Korawit Opassathian, Jan Hejátko, Stéphanie Robert, Jiří Friml, Karel Doležal, Karin Ljung, Colin Turnbull
Institutions: Swedish University of Agricultural Sciences, Umeå Plant Science Centre, Imperial College London, Czech Academy of Sciences, Institute of Experimental Botany, Palacký University Olomouc, Central European Institute of Technology, Institute of Science and Technology Austria, Masaryk University, Central European Institute of Technology – Masaryk University, Centre of the Region Haná for Biotechnical and Agricultural Research, University of Copenhagen, Chr. Hansen (Denmark)
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,380
Funders: European Molecular Biology Organization, Institute of Science and Technology Austria, Company of Biologists, European Commission, Ministerstvo Školství, Mládeže a Tělovýchovy, Grantová Agentura České Republiky, VINNOVA, Austrian Science Fund, Fonds Wetenschappelijk Onderzoek, Knut och Alice Wallenbergs Stiftelse, Vetenskapsrådet, Central European Institute of Technology, Univerzita Palackého v Olomouci, Kempestiftelserna, European Regional Development Fund, H2020 European Research Council
Awards: G1059663884, G12500569, G140558338, Tracing Evolution of Auxin Transport and Polarity in Plants, G1564352550, G176604203, G2060342649, G2061982546, Study of the intracellular distribution of cytokinins and their transport to vacuoles, G2693309452, G2775765354, G2876455165, G291036901, G330045347, G3490171961, G3496015395, G3498507180, G3622461675, G3664527855, G3826070532, G4131035492, G4272018831, G4280972364, G434337789, G4652765776, G5005566375, CEITEC 2020, Molecular mechanisms underlying cytokinin-mediated regulation of organ identity in Arabidopsis., G5641063287, G5773228896, G5916014575, G6231564467, G6747061678, G6875092472, G7363506711, G7475903712, G7593166679, G8376479305, Mode of action studies of a new plant growth activator, UPSC Berzelii Centre for Forest Biotechnology, G8841348983, G9000262362
OpenAlex ID: W3080154717