Work

Optimal correlation order in superresolution optical fluctuation microscopy

Year: 2020

Type: article

Abstract: Here, we show that superresolution optical fluctuation microscopy might not lead to ideally infinite superresolution enhancement with increasing of the order of measured cumulants. Using information analysis for estimating error bounds on the determination of point-source positions, we show that reachable precision per measurement might be saturated with increasing of the order of the measured cumulants in the superresolution regime. In fact, there is an optimal correlation order beyond which there is practically no improvement for objects of three and more point sources. However, for objects of just two sources, one still has an intuitively expected resolution increase with the cumulant order.

Source: Physical Review A

DOI: 10.1103/physreva.102.063507

Authors: S. Vlasenko, A. B. Mikhalychev, I. L. Karuseichyk, D. A. Lyakhov, D. L. Michels, D. Mogilevtsev

Institutions: National Academy of Sciences of Belarus, B.I. Stepanov Institute of Physics, King Abdullah University of Science and Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: King Abdullah University of Science and Technology

Award: G4358210664