A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites
Year: 2020
Type: article
Abstract: This study describes a simple method for the large-scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.
Source: Veterinary Medicine and Science
DOI: 10.1002/vms3.364
Authors: Saeed El‐Ashram, Yu Zhang, Yongsheng Ji, Dina Salama, Kun Mei, Li Zhili, Huang Shujian, Haoji Zhang, Shawky M. Aboelhadid, Reem A. Alajmi, Dina M. Metwally, Manal F. El‐Khadragy, Billy M. Hargis, Guillermo Tellez‐Isaias, Beniamino T. Cenci‐Goga, Musafiri Karama, Munyaradzi C. Marufu, Fathi Abouhajer, Gamal Ali Abdelhafez Hamady, Abeer El Wakil, Ibrahim Al Nasr, Xun Suo
Institutions: Kafrelsheikh University, Foshan University, University of Science and Technology of China, Beni-Suef University, National Research Centre, King Saud University, Helwan University, University of Arkansas at Fayetteville, University of Perugia, University of Pretoria, Alasmarya Islamic University, Al-Azhar University, Alexandria University, Qassim University, China Agricultural University
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,400
Funder: Foshan University
OpenAlex ID: W3089177748