Work

Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures

Year: 2020

Type: article

Abstract: The soil bacterium Burkholderia thailandensis produces 2-alkyl-4(1 H )-quinolones that are mostly methylated 4-hydroxyalkenylquinolines, a family of relatively unstudied metabolites similar to molecules also synthesized by Pseudomonas aeruginosa . Several of the methylated 4-hydroxyalkenylquinolines have antimicrobial activity against other species. We show that Bacillus subtilis strain 168 is particularly susceptible to N -oxidated methylalkenylquinolines (HMAQ-NOs). We confirmed that HMAQ-NO biosynthesis requires the previously unstudied protein HmqL. These results provide new information about the biology of 2-alkyl-4(1 H )-quinolones, particularly the methylated 4-hydroxyalkenylquinolines, which are unique to B. thailandensis . This study also has importance for understanding B. thailandensis secondary metabolites and has implications for potential therapeutic development.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.01452-20

Authors: Jennifer R Klaus, Charlotte Majerczyk, Stephanie Moon, Natalie A. Epplera, Sierra Smith, Emily Tuma, Marie-Christine Groleau, Kyle L. Asfahl, Nicole E. Smalley, Hillary S. Hayden, Marianne Piochon, Patrick Ball, Ajai A. Dandekar, Charles Gauthier, Eric Déziel, Josephine R. Chandler

Institutions: University of Kansas, Oregon State University Cascades, Institut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, University of Washington

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Applied Microbiology and Biotechnology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funders: Cystic Fibrosis Foundation, Kansas IDeA Network of Biomedical Research Excellence, Government of Canada, National Institutes of Health, Canadian Institutes of Health Research, Natural Sciences and Engineering Research Council of Canada, National Institute of General Medical Sciences

Awards: Quorum sensing evolution and function in mixed bacterial communities, G1507670593, Investigations on 4-hydroxy-2-alkylquinoline (HAQ) derivatives produced by Burkholderia and Pseudomonas, G2921881625, Pseudomonas aeruginosa quorum sensing, G4959470276, New approaches for the stereoselective synthesis of glycans of therapeutic interest, G5153375419, Molecular Analysis of Disease Pathways, G7285114270, Chemical Biology of Infectious Disease, Kansas IDeA Network of Biomedical Research Excellence 5

OpenAlex ID: W3089529641

FWCI: 1.133

Cites: 71

Cited by: 24

Related to: 10