Work

High-throughput Minitaturized RNA-Seq Library Preparation

Year: 2020

Type: article

Abstract: Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research. We present here an automated and miniaturized workflow for RNA library preparation that minimizes reagent usage and processing time required per sample to generate Illumina compatible libraries for sequencing. The reduced-volume libraries show similar behavior to full-scale libraries with comparable numbers of genes detected and reproducible clustering of samples.

Source: Journal of Biomolecular Techniques JBT

DOI: 10.7171/jbt.20-3104-004

Authors: Samuel Mildrum, Austin Hendricks, Alexei Stortchevoi, Noelani Kamelamela, Vincent L. Butty, Stuart S. Levine

Institution: Massachusetts Institute of Technology

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Institutes of Health, National Cancer Institute, National Institute of Environmental Health Sciences

Awards: MIT Center for Environmental Health Sciences, Cancer Center Support (Core) Grant