A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome
Year: 2020
Type: article
Abstract: The Casparian strip (CS) constitutes a physical diffusion barrier to water and nutrients in plant roots, which is formed by the polar deposition of lignin polymer in the endodermis tissue. The precise pattern of lignin deposition is determined by the scaffolding activity of membrane-bound Casparian Strip domain proteins (CASPs), but little is known of the mechanism(s) directing this process. Here, we demonstrate that Endodermis-specific Receptor-like Kinase 1 (ERK1) and, to a lesser extent, ROP Binding Kinase1 (RBK1) are also involved in regulating CS formation, with the former playing an essential role in lignin deposition as well as in the localization of CASP1. We show that ERK1 is localized to the cytoplasm and nucleus of the endodermis and that together with the circadian clock regulator, Time for Coffee (TIC), forms part of a novel signaling pathway necessary for correct CS organization and suberization of the endodermis, with their single or combined loss of function resulting in altered root microbiome composition. In addition, we found that other mutants displaying defects in suberin deposition at the CS also display altered root exudates and microbiome composition. Thus, our work reveals a complex network of signaling factors operating within the root endodermis that establish both the CS diffusion barrier and influence the microbial composition of the rhizosphere.
Source: Plant and Cell Physiology
DOI: 10.1093/pcp/pcaa170
Institutions: University of Warwick, University of Nottingham, Cluster of Excellence on Plant Sciences, Max Planck Institute for Plant Breeding Research, KU Leuven, The University of Tokyo, University of Oxford, HUN-REN Szegedi Biológiai Kutatóközpont, Institute of Plant Biology, Hungarian Academy of Sciences, National University of Singapore
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,085
Awards: 14 ERA-CAPS.EVOREPRO.Evolution of Sexual Reproduction in Plants, Roots of decline? Assembly and Function of the Rhizosphere Microbiome in Relation to Yield Decline, Integrated approaches to plant sexual reproduction studies to enhance food security, High Throughput Fluorescence Imaging for Plant Sciences, G5368808017, MYB36 controls differentiation of the endodermis into an ion-selective barrier, Understanding the formation and function of apoplastic barriers, 13 ERA-CAPS: Plant root diffusional barriers: Genesis and implications for nutrient efficiency and stress tolerance, Control of early embryogenesis by extra-embryonic peptides, G793617395, Regulation of the male germline in cereal crops by somatic companion cells
OpenAlex ID: W3122811161