Effect of uremic toxins on hippocampal cell damage: analysis in vitro and in rat model of chronic kidney disease
Year: 2021
Type: article
Abstract: One third of the patients with chronic kidney disease (CKD) develop cognitive impairment, which is also an independent risk factor for mortality. However, the concise mechanism of cerebro-renal interaction has not been clarified. The present study examines the effects of uremic toxins on neuronal cells and analyzes the pathological condition of the brain using mouse hippocampal neuronal HT-22 cells and adenine-induced CKD model rats. Among the uremic toxins analyzed, indoxyl sulfate, indole, 3-indoleacetate, and methylglyoxal significantly decreased viability and glutathione level in HT-22 cells. The mixture of these uremic toxins also decreased viability and glutathione level at a lower dose. Adenine-induced CKD rat showed marked renal damage, increased urinary oxidative stress markers, and increased numbers of pyknotic neuronal cells in hippocampus. CKD rats with damaged hippocampus demonstrated poor learning process when tested using the Morris water maze test. Our results suggest that uremic toxins have a toxic effect on hippocampal neuronal cells and uremic CKD rats shows pyknosis in hippocampus.
Source: Heliyon
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,750
Awards: Pathological involvement of carbonyl stress for development of cognitive impairment in CKD, Elucidating the pathogenic mechanism of CKD-associated cognitive dysfunction and muscle wasting as revealed by metabolic changes in organs, G5344601884, Chronic kidney disease (CKD) and Cognitive impairment: Possible mechanisms of Cerebro-renal interaction, Gut-renal axis: microbiota and chronic kidney disease
OpenAlex ID: W3127036141