Work

Hypoxia protects H9c2 cells against Ferroptosis through SENP1-mediated protein DeSUMOylation

Year: 2021

Type: article

Abstract: Hypoxia affects proliferation, differentiation, as well as death of cardiomyocyte, and plays an important role in the development of myocardial ischemia. However, the detailed mechanisms through which hypoxia regulates cardiomyocyte ferroptosis have not been explored. In this study, we revealed that hypoxia suppresses the proliferation, migration, and erastin-induced ferroptosis of H9c2 cells. First, we confirmed the upregulation of SENP1 in H9c2 cells cultured under hypoxic conditions. Through adenovirus-mediated SENP1 gene transfection, we demonstrated that SENP1 overexpression could enhance H9c2 cell proliferation and migration while also protecting H9c2 cells from erastin-induced ferroptosis. Furthermore, through immunoprecipitation and western blotting, we confirmed that SENP1 mediated deSUMOylation of HIF-1α and ACSL4 in H9c2 cells. In conclusion, this study describes the underlying mechanism through which hypoxia upregulates SENP1 expression, in turn protecting against ferroptosis via the regulation of HIF-1α and ACSL4 deSUMOylation. Our findings provide a theoretical foundation for the development of novel therapeutics for ischemic heart diseases.

Source: International Journal of Medical Sciences

DOI: 10.7150/ijms.50804

Authors: Yu-Ting Bai, Feng-Jun Xiao, Hua Wang, Ri-Li Ge, Li-Sheng Wang

Institutions: Qinghai University, Qinghai Provincial Peoples Hospital, Affiliated Hospital of Qingdao University

Language: English

Topic: Ferroptosis and cancer prognosis

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,337