Work

TLC directed isolation and in silico analysis of antimicrobial metabolite from Nigrospora sphaerica inhabiting Croton bonplandianus Baill

Year: 2021

Type: article

Abstract: TLC-bioautography amalgamated with hyphenated spectroscopy aid in precise in situ detection of secondary metabolites with pharmaceutical significance. TLC bioautography offers efficient and economical strategy in identifying compounds of interest from crude extracts. The present investigation has been focused on detection of antimicrobial metabolite from the culture broth of Nigrospora sphaerica inhabiting Croton bonplandianus Baill. The antimicrobial profiling confirmed the bioactive nigrosporalactone to possess broad-spectrum activity against test human pathogens with minimum inhibitory concentration values in the range 6.25 µg to 100 µg. The in silico studies revealed protein targets 1I01 (E. coli beta-ketoacyl reductase), 1IYL (C. albicans N-myristoyl transferase) had the highest binding score of -6.1 Kcal/mol.

Source: South African Journal of Botany

DOI: 10.1016/j.sajb.2021.01.035

Authors: N. Chandra Mohana, D. Rakshith, K.P. Ramesha, B.R. Nuthan, B.P. Harini, S. Satish

Institutions: University of Mysore, Bangalore University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Applied Microbiology and Biotechnology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,750