Work

16S Illumina Primer Plate Aliquoting (working plates 5µM Forw & Rev) v1

Year: 2018
Type: preprint
Abstract: The following protocol outlines aliquoting resuspended primers, stored at 100µM in stock plates, into working primer plates at a conentration of 5µM for both forward (barcoded) and reverse primers, using the epMotion 5075. The Knigh Lab stores primer combos premixed in working primer plates.
Language: English
Subfield: Ecology
Open Access status: green
OpenAlex ID: W3133825341
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