A novel polysaccharide from plant fermentation extracts and its immunomodulatory activity in macrophage RAW264.7 cells
Year: 2021
Type: article
Abstract: Plant fermentation extracts (PFEs) produced from fermenting various fruits and vegetables by microorganisms have traditionally been consumed as healthy beverages in Asia. Herein, the immunomodulatory activities of a novel polysaccharide (AP) isolated from PFE were examined in RAW264.7 cells. The results showed that AP was composed of Rha, Xyl, Man and Glu in a molar ratio of 9.7:27.6:8.81:53.85 and the average molecular weight was 4.81 × 105 Da. Our data showed that AP could significantly enhance the phagocytic activity of RAW264.7 cells and their ability to release NO, TNF-α, IL-6 and IL-10. Furthermore, AP could significantly up-regulate the mRNA expression of iNOS, cytokines (TNF-α, IL-6, IL-10) and pattern recognition receptors (TLR2, MR, GR, CR3 and SR). Western blotting demonstrated that the regulation of NO and TNF-α were mediated through the NF-κB and MAPK signalling pathways. These findings helped to elucidate the immunomodulatory properties of the polysaccharide AP and PFE.
Source: Food and Agricultural Immunology
Authors: Yan Liang, Chunhua Liu, Shuxia Yan, Pu Wang, Binbin Wu, Chengzi Jiang, Xiaoqing Li, Yanwen Liu, Xiang Li
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,306
OpenAlex ID: W3134594645