SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis
Year: 2021
Type: article
Abstract: There is ongoing debate as to whether cardiac complications of coronavirus disease-2019 (COVID-19) result from myocardial viral infection or are secondary to systemic inflammation and/or thrombosis. We provide evidence that cardiomyocytes are infected in patients with COVID-19 myocarditis and are susceptible to severe acute respiratory syndrome coronavirus 2 . We establish an engineered heart tissue model of COVID-19 myocardial pathology, define mechanisms of viral pathogenesis , and demonstrate that cardiomyocyte severe acute respiratory syndrome coronavirus 2 infection results in contractile deficits, cytokine production , sarcomere disassembly, and cell death. These findings implicate direct infection of cardiomyocytes in the pathogenesis of COVID-19 myocardial pathology and provides a model system to study this emerging disease.
Authors: Adam L. Bailey, Oleksandr Dmytrenko, Lina Greenberg, Andrea L. Bredemeyer, Pan Ma, Jing Liu, Vinay Penna, Emma S. Winkler, Sanja Sviben, Erin Brooks, Ajith P. Nair, Kent A. Heck, Aniket S. Rali, Leo Simpson, Mehrdad Saririan, Dan Hobohm, W. Tom Stump, James A. Fitzpatrick, Xuping Xie, Xianwen Zhang, Pei-Yong Shi, J. Travis Hinson, Weng-Tein Gi, Constanze Schmidt, Florian Leuschner, Chieh-Yu Lin, Michael S. Diamond, Michael J. Greenberg, Kory J. Lavine
Institutions: Washington University in St. Louis, University of Wisconsin–Madison, UW Health University Hospital, Baylor College of Medicine, Vanderbilt University, Creighton University, Valleywise Health, The University of Texas Medical Branch at Galveston, The Jackson Laboratory for Genomic Medicine, UConn Health, Heidelberg University, University Hospital Heidelberg
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,400
Funders: Burroughs Wellcome Fund, March of Dimes Foundation, Foundation for Barnes-Jewish Hospital, Children's Discovery Institute, St. Louis Children's Hospital, National Institutes of Health, Defense Advanced Research Projects Agency, Center for Cellular Imaging, Washington University, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases
Awards: G1037950686, Human antibody-based countermeasures against the Wuhan Coronavirus SARS-CoV-2, G1382039244, G1897615168, G2013629834, G2117646143, Regulation of Antiviral Responses in Plasmacytoid Dendritic Cells to Chikungunya Virus by the Gut Microbiota, G2437195121, G261815350, G2806300442, MACROPHAGE HETEROGENEITY IN HEART FAILURE PROGRESSION AND CARDIAC RECOVERY, G3168635882, Role of ontogeny and niche on human macrophage development, Human neutralizing antibodies for Zika virus, MACROPHAGE ONTOGENY SHIFTS IN ISCHEMIC HEART DISEASE, G4299108669, G4476186264, G4673675110, G5151536333, G5571277077, G63402963, G6698518189, National Research Service Award-Medical Scientist, G8164162099, Regulation of cardiac power output in health and disease, Training Program in Basic Immunology
OpenAlex ID: W3134776434