Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials
Year: 2021
Type: article
Abstract: Background: The potential applications of protein-engineered functional materials are so wide and exciting that the interest in these eco-friendly advanced materials will further expand in the future. Tag-mediated protein purification/immobilization technologies have emerged as green and cost-effective approaches for the fabrication of such materials. Strategies that combine the purification and immobilization of recombinant proteins/peptides onto/into natural, synthetic or hybrid materials in a single-step are arising and attracting increasing interest. Aim of Review: This review highlights the most significant advances of the last 5 years within the scope of tag-mediated protein purification/immobilization and elucidates their contributions for the development of efficient single-step purification and immobilization strategies. Recent progresses in the field of protein-engineered materials created using innovative protein-tag combinations and future opportunities created by these new technologies are also summarized and identified herein. Key Scientific Concepts of Review: Protein purification/immobilization tags present a remarkable ability to establish specific non-covalent/covalent interactions between solid materials and biological elements, which prompted the creation of tailor-made and advanced functional materials, and of next-generation hybrid materials. Affinity tags can bind to a wide range of materials (of synthetic, natural or hybrid nature), being most suitable for protein purification. Covalently binding tags are most suitable for long-term protein immobilization, but can only bind naturally to protein-based materials. Hybrid affinity-covalently binding tags have allowed efficient one-step purification and immobilization of proteins onto different materials, as well as the development of innovative protein-engineered materials. Self-aggregating tags have been particularly useful in combination with other tags for generating protein-engineered materials with self-assembling, flexible and/or responsive properties. While these tags have been mainly explored for independent protein purification, immobilization or functionalization purposes, efficient strategies that combine tag-mediated purification and immobilization/functionalization in a single-step will be essential to guarantee the sustainable manufacturing of advanced protein-engineered materials.
Source: Journal of Advanced Research
Authors: Ana I. Freitas, Lucília Domingues, Tatiana Q. Aguiar
Institution: University of Minho
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,200
Awards: G1167214205, Centro de Engenharia Biológica da Universidade do Minho, G1489525204, G1516346500, G1578237752, G1763632427, G274581676, BioTecNorte: Biotecnologia para o desenvolvimento sustentado da bioeconomia do Norte de Portugal, G3840324753, G3891694003, G4124477748, G4403996651, Bio-tintas fluorescentes fotocontroláveis, reversivelmente fotocomutáveis e eco-sustentáveis para o fabrico de têxteis inteligentes, G4850785371, G491858407, G5224927334, G5454278309, G6420836005, G6565430356, G7584003722, G7965899483
OpenAlex ID: W3176189464