Effect of solvents on metallic and phenolics content in buttercup tree bark vis-a- vis in relation to anthelmintic activity
Year: 2021
Type: article
Abstract: The aim of the present study to evaluate metal ion content and total phenolics in various extracts of Cochlospermum religiosum (CR) Alston using two different solvents.The CR is commonly known as Buttercup tree belongs to the family Bixaceae, having many branches.In the present study, ethylacetate, methanol and aqueous solvents are used for the extraction using microwave oven method.All the extracts are tested preliminary for the presence of phytochemicals, followed by TLC for identification.Furthermore, HPLC results estimated the gallic acid content is higher in methanol CR bark extract.The result revealed the presence of carbohydrates, alkaloids, glycosides, tannins, phenolics, flavonoids, and steroidal compounds in aqueous and methanol extracts whereas protein, lipids, and steroids are present in ethyl acetate extract.Content of metal ions (Fe, Mn, Cu, Zn, Co, Ni, As, Hg, Pb, Cd) are determined with atomic absorption spectrophotometer (AAS) and revealed the presence of higher amount of Zn (19.13 mg/g) with lower amount of Mn (0.06 mg/g) in methanol extract.Other heavy metals are present in negligible quantities.Furthermore, total phenolic content was higher in methanol extract (28.23 %) extracted for 10 min than other two extracts.Finally, in vitro anthelmintic activity of extracts was evaluated using reference to standard Albendazole and it showed significant results in concentration dependent manner against Pheretima posthuma, as test worm.The higher result was obtained with methanolic bark extract followed by aqueous extract (extracted with microwave oven for 10 min with 80 o C) and the result was due to presence of polyphenolic compound especially gallic acid (0.38 g), and also showed a positive correlation with yield and metal ion content.
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W3180971869