Spatiotemporal beam self-cleaning for high-resolution nonlinear fluorescence imaging with multimode fiber
Year: 2021
Type: article
Abstract: Beam self-cleaning (BSC) in graded-index (GRIN) multimode fibers (MMFs) has been recently reported by different research groups. Driven by the interplay between Kerr effect and beam self-imaging, BSC counteracts random mode coupling, and forces laser beams to recover a quasi-single mode profile at the output of GRIN fibers. Here we show that the associated self-induced spatiotemporal reshaping allows for improving the performances of nonlinear fluorescence (NF) microscopy and endoscopy using multimode optical fibers. We experimentally demonstrate that the beam brightness increase, induced by self-cleaning, enables two and three-photon imaging of biological samples with high spatial resolution. Temporal pulse shortening accompanying spatial beam clean-up enhances the output peak power, hence the efficiency of nonlinear imaging. We also show that spatiotemporal supercontinuum (SC) generation is well-suited for large-band NF imaging in visible and infrared domains. We substantiated our findings by multiphoton fluorescence imaging in both microscopy and endoscopy configurations.
Source: Scientific Reports
Institutions: Centre National de la Recherche Scientifique, XLIM, Université de Limoges, ALPhANOV (France), Institute of Physical Chemistry, Polish Academy of Sciences, Sapienza University of Rome, University of Brescia, Université de Bourgogne, Institut Universitaire de France, Laboratoire Interdisciplinaire Carnot de Bourgogne, Università Cattolica del Sacro Cuore
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,990
Awards: G1295597519, G1864783830, G2659019386, G3455407915, G3645878137, G434337789, G4656594441, G5321837785, G5630639691, Ingénierie et Innovation par les sciences physiques, les savoir-faire technologiques et l'interdisciplinarité, G6803667113, G7240651115, G7376787254, From specific ceramic materials and components to integrated, secured and intelligent communication systems, ISITE « BFC, G8232528393, G8371890388, elecTRo-optically Actuated all-FIber Components for advanced endoscopic system
OpenAlex ID: W3201600398