Work

No apparent p53 activation in CRISPR‐engineered gene‐edited rabbits

Year: 2021

Type: article

Abstract: Clustered regularly interspaced short palindromic repeats-CRISPR-associated 9 (CRISPR-Cas9) and base editors (BEs) are revolutionary gene-editing technology that has been widely utilized in biology, biotechnology and medicine. However, recent reports show that CRISPR-Cas9-mediated genome editing can induce a p53-mediated stress response and cell cycle arrest in human cells, while not illustrated in gene-editing animals. In the study, to verify whether there is a phenomenon of p53 activation, by analysing nine gene-edited rabbits using CRISPR-Cas9 and BEs, we provide the first evidence that no apparent p53 expression changes in those rabbits generated by Cas9 or BE-edited, suggesting that p53 may not need to consider for application in gene-edited animals.

Source: Journal of Cellular and Molecular Medicine

DOI: 10.1111/jcmm.16960

Authors: Tao Zhang, JinZe Li, Tian Wang, Feiyu Zhao, Tingting Sui

Institution: Jilin University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,388

Funders: People's Government of Jilin Province, National Key Research and Development Program of China, National Key Research and Development Program of China Stem Cell and Translational Research