Evaluation of Continuous Chemostat Cultivation of Thiobacillus Ferrooxidans on Ferrous Iron or Tetrathionate
Year: 1977
Type: conference-paper
Abstract: Thiobacillus ferrooxidans was cultured in continuous flow chemostats \non FeSO, at pHl.6 and K was subject to one or other of non-competitive product inhibition (NCP1) or \n254% at pH2.5. On iron as the limiting substrate, growth \npredominatly competitive product inhibition (PCP1). This resulted in premature \nwashout at dilution rates dependent on the concentration of FeSO, in the medium feed. \nGrowth yield, apparent maintenance energy requirement and maximum specific growth \nrate ae were determined over a wide range of dilution rates for cultures subject \nto either NCPl or PCP1l. With tetrathionate as the limiting substrate no inhibition \neffects were observed in the chemostat pH2.5, although tetrathionate is an inhibitory \nsubstrate at some pH values. Yield, apparent maintenance and ee page eater \nFor both substrates, true growth yield, Yo (g dry wt per g atom Fe or g mole \nKS 96 oxidized) was determined from the experimental data and enabled calculation \n24 \nof the Yarp (g dry wt per mole ATP available from each substrate) and the probable \n° + ° : . . . \nATP requirement for NAD reduction during iron oxidation.
Authors: D. P. Kelly, M. Eccleston, Carol Jones
Language: English
Subfield: Metals and Alloys
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3207365871