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Dried blood sample analysis by antibody array across the total testing process

Year: 2021
Type: article
Abstract: Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.
DOI: 10.1038/s41598-021-99911-8
Authors: Kelly Whittaker, Ying-Qing Mao, Yongping Lin, Huihua Zhang, Siwei Zhu, Hannah Peck, Ruo-Pan Huang
Institutions: RayBiotech (China), First Affiliated Hospital of Guangzhou Medical University, Georgia Institute of Technology, Emory University, The Wallace H. Coulter Department of Biomedical Engineering, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou Medical University
Language: English
Open Access status: gold
Estimated APC paid: $1,990
OpenAlex ID: W3207838239
FWCI: 0.699
Cites: 58
Cited by: 14
Related to: 10