Effect of subinhibitory concentrations of selected antibiotics and propolis on pyocyanin and biofilm production among Pseudomonas aeruginosa isolates in Alexandria, Egypt
Year: 2021
Type: article
Abstract: Background: Pseudomonas aeruginosa is a highly virulent microorganism that is implicated in various types of infections. It is armed with an arena of virulence factors that is mostly controlled by quorum sensing. The pigment pyocyanin and biofilm formation are of the important defense mechanisms used by the organism to establish infection. Objective: detection and quantification of biofilm mass and pyocyanin along with effect of subinhibitory concentrations of antibiotics on their magnitude. Methodology: In the present study, fifty Pseudomonas aeruginosa isolates were obtained from clinical laboratories from all over Alexandria governorate, Egypt. The isolates were tested for certain quorum sensing dependent virulence factors and the effect of subinhibitory concentrations of certain antibiotics, in addition propolis extract, was assayed. Results: the sub MIC of selected antibiotics and propolis inhibited pyocyanin production. On the other hand, they had variable effects on the formed biofilm mass. Conclusion: the effect of sub inhibitory concentrations either on biofilm formation or pyocyanin production is very important to be tested and followed to predict bacterial behavior and assist in tailoring therapeutic regimen.
Author: Ghada Ali
Institution: Pharos University in Alexandria
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W3207992196