Work

Novel Photothermal Aptamer-based Sensing System for Highly Ultrasensitive Detection of Ochratoxin A

Year: 2021

Type: article

Abstract: We developed a photothermal sensing system based on the photothermal effect of the Tetramethyl benzidine/Hydrogen Peroxide (TMB-H 2 O 2 ) colorimetric system mediated by Fe 3 O 4 nanoparticles.The ochratoxin A (OTA) aptamer can bind OTA particles with high affinity and specificity.The charge transfer complex of the single electron oxidation product of TMB (oxidized TMB) acts as a photothermal probe to convert the recognition signal into heat, allowing sensitive and quantitative photothermal detection using only a thermometer.In the proposed sensing system, the temperature change varied with the concentration of OTA.The aptamer-based sensing system provides a wide detection range for OTA from 0.25 nM to 2500 μM.We provide a new approach for the detection of mycotoxins because of the advantages of the simple readout method, low sample consumption, and lower detection cost.

Source: Sensors and Materials

DOI: 10.18494/sam.2021.3589

Authors: Pinzhu Qin, Ying Guan, Yige Qiu

Institution: Jiangsu Open University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $748

Funder: Government of Jiangsu Province