Serum apolipoprotein A-I potentiates the therapeutic efficacy of lysocin E against Staphylococcus aureus
Year: 2021
Type: article
Abstract: Lysocin E is a lipopeptide with antibiotic activity against methicillin-resistant Staphylococcus aureus . For unclear reasons, the antibacterial activity of lysocin E in a mouse systemic infection model is higher than expected from in vitro results, and the in vitro activity is enhanced by addition of bovine serum. Here, we confirm that serum from various species, including humans, increases lysocin E antimicrobial activity, and identify apolipoprotein A-I (ApoA-I) as an enhancing factor. ApoA-I increases the antibacterial activity of lysocin E when added in vitro, and the antibiotic displays reduced activity in ApoA-I gene knockout mice. Binding of ApoA-I to lysocin E is enhanced by lipid II, a cell-wall synthesis precursor found in the bacterial membrane. Thus, the antimicrobial activity of lysocin E is potentiated through interactions with host serum proteins and microbial components.
Source: Nature Communications
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,560
Funders: Takeda Science Foundation, Japan Agency for Medical Research and Development, Tokyo Biochemical Research Foundation, Japan Society for the Promotion of Science London, Ministry of Education, Culture, Sports, Science and Technology, Mochida Memorial Foundation for Medical and Pharmaceutical Research, Japan Society for the Promotion of Science
Awards: Functional analysis of novel substances from food materials showing strong abilities of innate immunity stimulation, Comprehensive analysis of pathogenic ncRNA in host environment, Mechanism of action analysis of novel antibiotics in host factor contribution to therapeutic efficacy, G2788000271, Comprehensive understanding of the innate immune system using the silkworm model, G3937311131, G4084956328, Platform for the identification of novel therapeutically effective antimicrobial agents, G4451709610, G4943066073, Establishment of a screening system for therapeutically effective antibiotics, Identification and characterization of novel virulence factors of Staphylococcus aureus USA300 using silkworm model., G5337109008, G6021262162, Expanding the medicinally relevant chemical space with architecturally complex natural products and their synthetic analogues, G7124806556, G7401522178, Structural Control and Generation of New Functional Molecules Using a Solid-Phase Library Strategy for Peptidic Natural Products, G8333284265
OpenAlex ID: W3209427366