Resveratrol antiaging impact on bone marrow-derived mesenchymal stem cells in a duration-dependent manner.
Year: 2021
Type: article
Abstract: Background: Aging is a natural life event associated with progressive cellular and biological dysfunction. It is a cumulative process that ends with impairment of the general function of the biological system. Stem cells are unique cells with behavioral diversity making them intriguing candidates for regenerative therapy.Resveratrol [RSV], a nonflavonoid polyphenol phytoalexin with a stilbene structure, was first extracted from the root of white hellebore, and it was widely used in traditional Chinese medicine. Materials And Methods: We achieved our study on bone marrow-derived mesenchymal stem cells [BM-MSCs] derived from 15-month aged rats. A total number of 40 female rats weighting [200-300g]. All animal procedures were approved by the Institutional Ethics Committee of Assiut University. Every attempt was made to minimize animal affliction. Resveratrol was given orally [through an intragastric tube] daily at a dose of 2.5 mg/kg diluted in 0.5ml phosphate buffer salineAnimals were subdivided into four equal subgroups; [A] control, [B] received resveratrol for one week, [C] received resveratrol for two weeks, and [D] received resveratrol for one month. Morphological changes were tracked, surface marker expression was measured, and various parameters of proliferation kinetics were used; colony-forming unit [CFU], population doubling time [PDT] "3‐[4,5‐Dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide [MTT]", "DNA index" and budding uninhibited by benzimidazole-related 1. [BubR1] immunocytochemical staining were implemented. Results: We reached significant results in improving morphological changes, proliferation kinetic parameters, immunophenotypic flowcytometric CD 90 marker expression, DNA index, and anti BubR1antibody immunocytochemical staining even after two weeks of treatment.Conclusions: Resveratrol had flourishing effects on MSC on aged cells. It promoted growth, proliferation, improved viability, established genetic stability, and improved MSC aging parameters documented in marked duration-dependent patterns.
Institution: Assiut University
Language: English
Subfield: Geriatrics and Gerontology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W3213068978