Work

Adjunctive viral cell culture supports treatment decision‐making in patients with secondary humoral immunodeficiency and persistent SARS‐CoV‐2 infection

Year: 2021

Type: article

Abstract: Single nucleotide polymorphisms (SNPs) in the B.1.177.5 SARS-CoV-2 lineage over time pre-and post-challenge with remdesivir. Wholegenome sequencing (WGS) was conducted as described in the Supplementary information. Post-sequencing analysis was conducted through CIVET version (v) 2.0, utilising several software packages (Python v3.6.13, Matplotlib v3.3.4, Pandas v1.1.0, Tabulate v0.8.9, CSV v1.0, Numpy v1.19.5, Scipy v1.5.3). Baltic COVID-19 Genomics data from 03 May 2021 were used as background data. Three SNPs were observed between sample 1 (day 1) and sample 2 (day 63) taken pre-remdesivir while a further five SNPs were observed between sample 2 (day 63, pre-remdesivir) and sample 3 (day 86, post-remdesivir). WGS was unable to identify three nucleotides on the day 63 sample (denoted as? or N), and unable to identify two nucleotides on the day 86 sample. [Colour figure can be viewed at wileyonlinelibrary.com

Source: British Journal of Haematology

DOI: 10.1111/bjh.17957