The use of solution NMR labeling techniques in solving structures of membrane proteins by solid state in-cell NMR
Year: 2013
Type: dissertation
Abstract: Solid state in-cell NMR is an excellent technique to study membrane proteins. The in-cell aspect resolves the requirement of solubilization and reconstitution steps, resulting in a far more straightforward expression scheme. And the solid state aspect removes the need of fast tumbling membrane proteins. In this study different labeling schemes are investigated for their use for structural assignment of membrane proteins by in-cell solid state NMR. By combining clever labeling schemes with in-cell solid state NMR, this technique can prove to be very useful in filling in the large gap of structural data for membrane proteins.
Author: N. Remers
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Eliminating Critical Systematic Errors In Structural Biology With Next-Generation Simulation, G4407949172, Maintenance of resistance genes in Golden Staph, Antibiotic resistance in hospital strains of Golden Staph, ALS Efficiently Networking Advanced Beam Line Experiments (ALS-ENABLE), Deciphering fundamental biological processes involving protein-nucleic acid interactions at the molecular level
OpenAlex ID: W35907571