HLA Class I and Class II Antigen Expression in Gastric Cancer Cells and Induction of Its Expression by Local Injection of OK-432
Year: 1993
Type: article
Abstract: The measurements of HLA class I and II antigen expres-sions were made in association with assessment of antipro-liferation effects on cancer cells. Experiments were designed to measure HLA class I and II expressions by using flow cytometry in the mixed culture of WiDr cells, OK-432 and peripheral lymphocytes. The result showed that proliferation of cancer cells was suppressed in contact with OK-432, indicating augmented HLA class I and II antigen expression. It was suggested of activation of immunocompetent cells as well as induction of HLA antigen expression. HLA class I and II antigen expressions were clinically eveluated in patients with gastric cancer between with and with-out local administration of OK-432. Cancer tissues were fixed by to AMeX method and immunohisto-chemically stained by ABC method. The degrees of HLA class I antigen expressions were depressed in accordance with cancer progression. In contrast, hey were seen in the majority of patients in this study. On the other hand, HLA class II antigen expressions were reduced (p < 0.05) with cancer progression, the increase in cancer infiltration and the decrease in differentiation. It was suggested that reduction of HLA-class II (DR) antigen expression be in association with biologically malignant potential. In addition, it was assumed that local administration of OK-432 enabled HLA class II antigen expression and lymphocyte infiltration around the tumor to enhance ven in patients with advanced and undifferentiated cancers and it was suggested that OK-432 made it possible to induce tumor immunity and to become a valid method of postop-erative immunotherapy.
Author: Daikichi Okada
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W367359826