Selection of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone 10 by a Cell Cloning Technique
Year: 1981
Type: article
Abstract: Strains producing high levels of ubiquinone 10 were isolated from tobacco cell suspension cultures by a cell cloning technique, and the UQcontent of these cell strains was analyzed by high performance liquid chromatography.Three strains with high UQyields in suspension culture were selected and used for investigations on the necessary cultural conditions for UQproduction.Sucrose concentration, 2,4-d concentration and culture temperature did not have marked effects on UQ formation.These results differed from those of the original cell lines.Furthermore, the addition of yeast extract to the mediumpromoted cell growth in these strains remarkably but did not enhance UQ production in the cell strains.According to these investigations, UQproduction was increased to 15mg/liter and the UQlevel to 1890/^g/g dry wt. of cells after 1 1 days of incubation.
Authors: Takashi Matsumoto, Naomi Kanno, Tsutomu Ikeda, Yukiteru Obi, Takuro Kisaki, Masao Noguchi
Institutions: Central Research Institute, Japan Tobacco (Japan)
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W370774085