Repetitive system of direct regeneration of soybean (Glycine max (L.) Merr) from hypocotyl explants
Year: 2008
Type: article
Abstract: In vitro culture establishment, shoot proliferation and root development responses of soybean (Glycine max) were examined using various combinations of growth regulators. Hypocotyl and cotyledon explants were taken of which hypocotyl explants initiated on Muragshige and Skoog (MS) solid medium supplemented with 2mg L-1 2, 4-dichlorophenoxyacetic acid (2, 4-D) was the best for production of callus and with 2 mg L-1 of 6-benzylaminopurine (BAP) and 0.1 mg L-1 napthalene acetic acid (NAA) for shoot proliferation. Cotyledon explants inoculated remained dormant in the different combinations tried. Plants were rooted on half strength MS medium supplemented with 0.2 mg L-1 NAA. The regenerated plants grew normally in the greenhouse
Source: Madras Agricultural Journal
Authors: ARVINTH S, ARUN S, SELVAKESAVAN R.K
Institution: Sugarcane Breeding Institute
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W414779685