Transcriptomic adaptation during skeletal muscle habituation to eccentric or concentric exercise training
Year: 2021
Type: article
Abstract: Eccentric (ECC) and concentric (CON) contractions induce distinct muscle remodelling patterns that manifest early during exercise training, the causes of which remain unclear. We examined molecular signatures of early contraction mode-specific muscle adaptation via transcriptome-wide network and secretome analyses during 2 weeks of ECC- versus CON-specific (downhill versus uphill running) exercise training (exercise 'habituation'). Despite habituation attenuating total numbers of exercise-induced genes, functional gene-level profiles of untrained ECC or CON were largely unaltered post-habituation. Network analysis revealed 11 ECC-specific modules, including upregulated extracellular matrix and immune profiles plus downregulated mitochondrial pathways following untrained ECC. Of 3 CON-unique modules, 2 were ribosome-related and downregulated post-habituation. Across training, 376 ECC-specific and 110 CON-specific hub genes were identified, plus 45 predicted transcription factors. Secreted factors were enriched in 3 ECC- and/or CON-responsive modules, with all 3 also being under the predicted transcriptional control of SP1 and KLF4. Of 34 candidate myokine hubs, 1 was also predicted to have elevated expression in skeletal muscle versus other tissues: THBS4, of a secretome-enriched module upregulated after untrained ECC. In conclusion, distinct untrained ECC and CON transcriptional responses are dampened after habituation without substantially shifting molecular functional profiles, providing new mechanistic candidates into contraction-mode specific muscle regulation.
Source: Scientific Reports
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,990
Awards: G1108382433, G1316832455, G1365828906, G1633052362, G1778578463, G2005206474, G2055433861, G2432555772, G2655102363, G3113879043, Harnessing muscle-specific atrophy susceptibility to disentangle the mechanisms of disuse atrophy in human skeletal muscle, Examining the impact of inactivity and diet on muscle health with age in C. elegans, G3874286286, G3911965767, G4015916026, Establishing molecular mechanisms of and countermeasures to muscle decline in space, South West Biosciences: A Doctoral Training Programme for Bioscience students at Bristol, Bath, Cardiff, Exeter and Rothamsted Research, G5119652034, Centre for Musculoskeletal Ageing Research, Structural and metabolic determinants of sarcopenia and efficacy of concentric vs. eccentric exercise training: a novel temporospatial approach, New bioinformatics tools for novel fungicide target discovery, A scoping exercise and methods exchange in relation to differential muscle loss with disuse, Are mechanisms of skeletal muscle atrophy simply the reverse of muscle hypertrophy across age? A multi-omics approach, G657094295, CMAR Students, BB/J014400/1, G7815188220, G7895484610, G8022279748, Network analysis of human skeletal muscle adaptation under different loading states, G932456704
OpenAlex ID: W4200210878