Nasopharyngeal metatranscriptome profiles of infants with bronchiolitis and risk of childhood asthma: a multicentre prospective study
Year: 2021
Type: article
Study design: Observational Study
Abstract: Background Bronchiolitis is not only the leading cause of hospitalisation in US infants but also a major risk factor for asthma development. Growing evidence supports clinical heterogeneity within bronchiolitis. Our objectives were to identify metatranscriptome profiles of infant bronchiolitis, and to examine their relationship with the host transcriptome and subsequent asthma development. Methods As part of a multicentre prospective cohort study of infants (age <1 year) hospitalised for bronchiolitis, we integrated virus and nasopharyngeal metatranscriptome (species-level taxonomy and function) data measured at hospitalisation. We applied network-based clustering approaches to identify metatranscriptome profiles. We then examined their association with the host transcriptome at hospitalisation and risk for developing asthma. Results We identified five metatranscriptome profiles of bronchiolitis (n=244): profile A: virus RSV microbiome commensals ; profile B: virus RSV/RV-A microbiome H.influenzae ; profile C: virus RSV microbiome S.pneumoniae ; profile D: virus RSV microbiome M.nonliquefaciens ; and profile E: virus RSV/RV-C microbiome M.catarrhalis . Compared with profile A, profile B infants were characterised by a high proportion of eczema, Haemophilus influenzae abundance and enriched virulence related to antibiotic resistance. These profile B infants also had upregulated T-helper 17 and downregulated type I interferon pathways (false discovery rate (FDR) <0.005), and significantly higher risk for developing asthma (17.9% versus 38.9%; adjusted OR 2.81, 95% CI 1.11–7.26). Likewise, profile C infants were characterised by a high proportion of parental asthma, Streptococcus pneumoniae dominance, and enriched glycerolipid and glycerophospholipid metabolism of the microbiome. These profile C infants had an upregulated RAGE signalling pathway (FDR <0.005) and higher risk of asthma (17.9% versus 35.6%; adjusted OR 2.49, 95% CI 1.10–5.87). Conclusions Metatranscriptome and clustering analysis identified biologically distinct metatranscriptome profiles that have differential risks of asthma.
Source: European Respiratory Journal
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: Airway dual-transcriptomics in bronchiolitis and risk of asthma: MARC-35 cohort, Nasal microRNA during bronchiolitis and age 6y asthma phenotypes: MARC-35 cohort, Host genetics, early-life microbiome, and childhood asthma: MARC-43 Boston, Infant blood epigenome and risks of IgE sensitization, obesity, and asthma: MARC-35/43 cohorts, Infant specific-IgE, rhinovirus-C bronchiolitis, and incident asthma in MARC-35, G6465909309, Viral persistence & the microbiome in bronchiolitis and risk of recurrent wheeze, Airway metagenome & metabolome in bronchiolitis and risk of asthma: MARC-35 cohort, Prospective Cohort Study of Severe Bronchiolitis and Risk of Recurrent Wheezing, Clinical and Translational Science Institute at Childrens National, Host genetics, early-life microbiome, and childhood asthma: MARC-43 Boston, Interactions Between the Microbiome, Immunity and Respiratory Health in Traditional Agrarian and Suburban children, Integrating the genome, metabolome, and microbiome for childhood asthma: Risk and endotypes
OpenAlex ID: W4200532808