Work

Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells

Year: 2021

Type: article

Abstract: BACKGROUND/AIMSThe aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CD133þ Colo-320) or nonstem (CD133-Colo-320) cells. MATERIAL and METHODST. capitatus essential oil was obtained by water distillation and analyzed by GC-MS.Cancer stem cells (CD133þ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system.Proapoptotic activity of T. capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTSCaspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours.Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CD133þ and CD133-Colo-320 cells. CONCLUSIONWe conclude that T. capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis.Interestingly, T. capitatus essential oil is found to be more effective in Colo-320 cells than CD133þ Colo-320 and CD133-Colo-320 cells in terms of apoptosis.

Source: Cyprus Journal of Medical Sciences

DOI: 10.5152/cjms.2021.2293

Authors: Dudu Özkum Yavuz, Eda Becer, Remziye Kendirci, Feyzan Özdal Kurt, H. Seda Vatansever

Institutions: Near East University, Manisa Celal Bayar University

Language: English

Topic: Essential Oils and Antimicrobial Activity

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Anadolu Üniversitesi