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NAD + ameliorates endotoxin‐induced acute kidney injury in a sirtuin1–dependent manner via GSK‐3β/Nrf2 signalling pathway

Year: 2022

Type: article

Abstract: Acute kidney injury (AKI) is a substantial worldwide public health concern with no specific and effective therapies in clinic. NAD + is a pivotal determinant of cellular energy metabolism involved in the progression of AKI; however, its mechanism in kidney injury remains poorly understood. Sirtuin 1 (SIRT1) is an NAD + ‐dependent deacetylase associated with renal protection and acute stress resistance. In this study, we have investigated the role of NAD + in AKI and the potential mechanism(s) involved in its renoprotective effect. NAD + was notably decreased and negatively correlated with kidney dysfunction in AKI, restoring NAD + with NMN significantly ameliorates LPS‐induced oxidative stress and apoptosis and attenuates renal damage. We also found that the protection of NAD + is associated with SIRT1 expressions and performs in a SIRT1‐dependent manner. Inhibition of SIRT1 blunted the protective effect of NAD + and up‐regulated the activity of glycogen synthase kinase‐3β (GSK‐3β) that was concomitant with mitigated Nrf2 nuclear accumulation, thereby exacerbates AKI. These findings suggest that NAD + /SIRT1/GSK‐3β/Nrf2 axis is an important mechanism that can protect against AKI which might be a potential therapeutic target for the treatment of AKI.

Source: Journal of Cellular and Molecular Medicine

DOI: 10.1111/jcmm.17222