Year: 2022
Type: article
Abstract: Spatial omics emerged as a new frontier of biological and biomedical research. Here, we present spatial-CUT&Tag for spatially resolved genome-wide profiling of histone modifications by combining in situ CUT&Tag chemistry, microfluidic deterministic barcoding, and next-generation sequencing. Spatially resolved chromatin states in mouse embryos revealed tissue-type-specific epigenetic regulations in concordance with ENCODE references and provide spatial information at tissue scale. Spatial-CUT&Tag revealed epigenetic control of the cortical layer development and spatial patterning of cell types determined by histone modification in mouse brain. Single-cell epigenomes can be derived in situ by identifying 20-micrometer pixels containing only one nucleus using immunofluorescence imaging. Spatial chromatin modification profiling in tissue may offer new opportunities to study epigenetic regulation, cell function, and fate decision in normal physiology and pathogenesis.
Source: Science
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Yale University, Li Ka Shing Foundation, Society for Immunotherapy of Cancer, National Institutes of Health, Stand Up To Cancer, National Institute of Mental Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Advancing Translational Sciences
Awards: G1044130170, G143768663, High-spatial-resolution ECM-inclusive multi-omics sequencing of human PFA and FFPE tissue slides, High-spatial-resolution ECM-inclusive multi-omics sequencing of human PFA and FFPE tissue slides, G3473986544, G499332564, G591288840, G895817215, G9000152884, G9000159197, G9000196333
OpenAlex ID: W4211162540