Year: 2022
Type: article
Abstract: The Wnt/β-catenin pathway is involved in development, cancer, and embryonic stem cell (ESC) maintenance; its dual role in stem cell self-renewal and differentiation is still controversial. Here, by applying an in vitro system enabling inducible gene expression control, we report that moderate induction of transcriptionally active exogenous β-catenin in β-catenin null mouse ESCs promotes epiblast-like cell (EpiLC) derivation in vitro . Instead, in wild-type cells, moderate chemical pre-activation of the Wnt/β-catenin pathway promotes EpiLC in vitro derivation. Finally, we suggest that moderate β-catenin levels in β-catenin null mouse ESCs favor early stem cell commitment toward mesoderm if the exogenous protein is induced only in the "ground state" of pluripotency condition, or endoderm if the induction is maintained during the differentiation. Overall, our results confirm previous findings about the role of β-catenin in pluripotency and differentiation, while indicating a role for its doses in promoting specific differentiation programs.
Source: iScience
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,000
Funders: Università degli Studi di Napoli Federico II, University of Bristol, Fondazione Telethon, Horizon 2020 Framework Programme, HORIZON EUROPE Framework Programme, Medical Research Council, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, BrisSynBio
Awards: G1280652415, BrisSynBio: Bristol Centre for Synthetic Biology, G2218306764, G396232211, G4494338184, G5045220161, G5133049693, G552431277, G5894918726, G621933505, G6247987398, G6891521090, G7045001909, AUTOMATIC CELL FATE ENGINEERING USING MICROFLUIDICS DEVICES, COMBO: CONTROL-BASED BIODESIGN OF MAMMALIAN CELL DYNAMICS, Unravelling the role of beta-catenin in ground state pluripotency
OpenAlex ID: W4223412964