Controlled Formation of Emissive Gold Nanoclusters with a Synthetic Self-Assembled Protein Toroid
Year: 2022
Type: preprint
Abstract: Self-assembled proteins are privileged building blocks for the bottom-up organization of matter at the nanoscale. However, since most proteins are very large, they have to be produced by recombinant expression, which is less versatile and flexible than chemical synthesis. Here, we show that we can bridge the potential of proteins for nanofabrication with the simplicity and versatility of solid-phase peptide synthesis by relying on the self-assembly of the viral protein gp23.1, a small 50-residue protein that oligomerizes in solution to form a stable toroidal hexamer. We report the chemical synthesis and basic biophysical characterization of a gp23.1 mutant and show that its self-assembled hexamer templates the formation of highly monodisperse luminescent gold nanoclusters of about 1.3 nm inside its central cavity. This work demonstrates the versatility of this small self-assembled ring protein for a variety of nanotechnological applications.
Source: ChemRxiv
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Funders: European Commission, Xunta de Galicia, Centro Singular de Investigación de Galicia, National Institutes of Health, University of California, San Francisco, Agència de Gestió d'Ajuts Universitaris i de Recerca, Consejo Superior de Investigaciones Científicas, European Regional Development Fund, Agencia Estatal de Investigación, NIH Office of the Director, National Institute of General Medical Sciences
Awards: G1218400627, G1483635673, G1496055848, G1503431219, G1535402598, G167218828, G1869016439, G2261121936, G2538972755, G2845908698, G3113808516, G3372464033, Resource for Biocomputing Visualization and Informatics, G3528345224, G3550760735, G3694284866, G3852777410, G3857021370, G3876637791, G3987574874, G4180404498, G425571989, G4264401571, G4364831979, G4665235069, G4815039349, G4912030886, G540918280, G5554424168, G5823622512, G6141711610, G6298273575, G6332252478, G692825579, G7125151478, G7354949293, G7450264098, G7546465473, G78298304, G814220960, G947703345
OpenAlex ID: W4224255590