Year: 1985
Type: article
Abstract: The cell differentiation system of Friend leukemia cells was applied to screening for new types of antitumor antibiotics.F5-5, Friend leukemia cells, were the most suitable for the assay system due to the stability of their response on repeated culture passages.Antibiotics like mitomycin C, adriamycin and actinomycin D, but not cycloheximide, did not induce detectable benzidine-positive cells among the F5-5 cells in the concentration ranges tested.Among the culture fluids of one thousand and fifty-one streptomycete strains subjected to the assay system, actinomycin V, FL-51 8 and FL-657 were found to be the most active as inducers.Actinomycin V possessing l-4ketoproline as a substitute for L-proline of actinomycin D at a concentration of l.Ong/ml caused 39.7% of the F5-5 cells to become benzidine-positive.Furthermore, actinomycin V inhibited the colony formation of F5-5 cells in the soft agar mediumat a concentration of 0.004ng/ml.
Institution: Ajinomoto (Japan)
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4237027675