Year: 2021
Type: article
Abstract: East Java and has several medical functions.[21][22][23] C. cainito leaves contain compounds such as phenol, alkaloid, isoflavonoid, sterol, and triterpenoid.24 Both isoflavones or sterols are known as phytoestrogens due to their similar structure with 17β-estradiol.25 Sex hormones, such as estrogen and testosterone have crucial roles in maintaining bone homeostasis, both in women and men.There is a relationship between bone density with estrogen concentration in women or testosterone in men.26 There is a possibility that phytoestrogens in C. cainito can also act as phytotestosterone.This study aimed to identify the activity of 96% ethanol extract of C. cainito leaves in increasing the bone density of trabecular vertebra bone of dexamethasone-induced male mice (Mus musculus).The analysis was to predict the phytoestrogen compounds using in silico study and then analyze with molecular docking, one of the most frequently used methods in structure-based drug design.This method can predict the binding-conformation of small molecular ligands.27,28 The active site of the protein was firstly identified as the binding site.It uses interacting molecules to decide the signal-transduction as agonism or antagonism.29,30 This was conducted with a view to developing new drugs from natural ingredients.C. cainito has a potential as an anti-osteoporosis agent in postmenopausal women with minimum side effects. Materials and MethodsPlant materials C. cainito leaves were obtained and identified in UPT Materia Medica, Batu, Indonesia, in October 2017 with specimen number 1b- 2b-3b-4b-5b-6b-7b-8b-9b-10b-11b-12b-13b-14a-15a-109b-119b-120a-121b-124b-125a-126b-127a.The leaves were dried and ground to produce dry powder of C. cainito leaves.
Authors: Riwanti, Pramudita, Arifin, Miftah S., Muslikh, Faisal A., Amalia, Dilla, Abada, Iffatul, Aditama, Agnis P., Ma’arif, Burhan
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4242460179