A Versatile and Scalable Two-Step Ion-Exchange Chromatography Process for the Purification of Recombinant Adeno-associated Virus Serotypes-2 and -5
Year: 2002
Type: article
Abstract: Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and-5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such,this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models.
Source: Molecular Therapy
Authors: Nicole Brument, Robert Morenweiser, Véronique Blouin, Estelle Toublanc, Isabelle Raimbaud, Yan Chérel, Sébastien Folliot, Florence Gaden, Pierre Boulanger, Gabrielle Kroner-Lux, Philippe Moullier, Fabienne Rolling, Anna Salvetti
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4245087155