Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures.
Year: 2011
Type: article
Abstract: Gephyrin Regulates GABAergic and Glutamatergic Synaptic Transmission in Hippocampal Cell CulturesJournal of Biological ChemistryVol. 286Issue 23PreviewGephyrin is a scaffold protein essential for stabilizing glycine and GABAA receptors at inhibitory synapses. Here, recombinant intrabodies against gephyrin (scFv-gephyrin) were used to assess whether this protein exerts a transynaptic action on GABA and glutamate release. Pair recordings from interconnected hippocampal cells in culture revealed a reduced probability of GABA release in scFv-gephyrin-transfected neurons compared with controls. This effect was associated with a significant decrease in VGAT, the vesicular GABA transporter, and in neuroligin 2 (NLG2), a protein that, interacting with neurexins, ensures the cross-talk between the post- and presynaptic sites. Full-Text PDF Open Access VOLUME 286 (2011) PAGES 20942–20951 PAGE 20949: Lines 16 and 17 under “Discussion” should read “by a clear increase in the number of transmitter failures and in the PPR … ”
Source: Journal of Biological Chemistry
Authors: Zeynep Kasap Varley, Rocco Pizzarelli, Roberta Antonelli, Stefka H. Stancheva, Matthias Kneussel, Enrico Cherubini, Paola Zacchi
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W4254934232