Work

Protocol for binary food choice assays using Drosophila melanogaster

Year: 2022

Type: article

Abstract: Food preference is a fundamental behavior for animals to choose nutritious foods while rejecting foods containing toxins. Here, we describe binary food choice assays using Drosophila melanogaster, which are straightforward approaches for the characterization of two-way choice tastants. We detail the preparation of flies and dye-containing food, followed by the binary-choice feeding assays and the determination of the preference index (PI). This protocol is simple, sensitive, and reproducible in qualitatively detecting attractive or aversive characteristics toward any two-way choice tastants. For complete details on the use and execution of this protocol, please refer to Aryal et al. (2022).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101410

Authors: Binod Aryal, Subash Dhakal, Bhanu Shrestha, Jiun Sang, Roshani Nhuchhen Pradhan, Youngseok Lee

Institution: Kookmin University

Language: English

Topic: Neurobiology and Insect Physiology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500