Work

Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome

Year: 2022

Type: article

Abstract: An induced pluripotent stem cell (hiPSC) line (MPIi008-A) was generated from fibroblasts of a 1-year-old male patient with Denys-Drash syndrome using lentiviral delivery of reprogramming factors OCT4, SOX2, KLF4 and c-MYC. The MPIi008-A iPSC line exhibited typical iPSC morphology and normal karyotype, expressed pluripotent stem cell markers, and showed developmental potential to differentiate into derivatives of all three germ layers in vivo. The hiPSC line harbours a heterozygous missense mutation (R394L) in exon 9 of the WT1 gene.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2022.102826

Authors: Markus C. Doeser, Julia Krygin, Albrecht Röpke, Dong Han, Roland Wedlich-Söldner, Hans R. Schöler, Hermann Pavenstädt, Kee-Pyo Kim

Institutions: University of Münster, University Hospital Münster, Max Planck Institute for Molecular Biomedicine, Catholic University of Korea

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,100

Funders: Deutsche Forschungsgemeinschaft, Catholic University of Korea, Ministry of Science, ICT and Future Planning, National Research Foundation of Korea, Interdisziplinäres Zentrum für Klinische Forschung, Universitätsklinikum Würzburg, Ministry of Science and ICT, South Korea

Awards: G246298408, G2646711713, G327913919, G3813495418, G5265197696, G7464617573

OpenAlex ID: W4281624865

FWCI:

Cites: 4

Cited by:

Related to: 10