Fine Mapping and Cloning of a Major QTL qph12, Which Simultaneously Affects the Plant Height, Panicle Length, Spikelet Number and Yield in Rice (Oryza sativa L.)
Year: 2022
Type: article
Abstract: Plant height is one of the most important agronomical traits in rice (Oryza sativaL.). Introducing thesemidwarfrice in the 1960s significantly enhanced the rice yield potential in Asia. Implementing near-isogenic lines (NILs) is the most powerful tool for the identification and fine mapping of quantitative trait loci (QTLs). In this study, 176 NILs were produced from the crossing and back-crossing of two rice cultivars. Specifically, theindicarice cultivar Jiafuzhan served as a recipient, and the restorerjaponicacultivar Hui1586 served as a donor. Using the 176 NILs, we identified a novel major QTL for reduced plant height in the NIL36 line. Theqph12QTL was mapped to a 31 kb genomic region between the indel markersIndel12-29andIndel12-31. The rice genome annotation indicated the presence of three candidate genes in this genomic region. Through gene prediction and cDNA sequencing, we confirmed thatLOC_Os12g40890(qPH12) is the target gene in the NIL36 line. Further analysis showed that theqph12QTL is caused by a 1 bp deletion in the first exon that resulted in premature termination of theqPH12. Knockout experiments showed that theqph12QTL is responsible for the reduced plant height phenotype of the NIL36 line. Although theqph12gene from the NIL36 line showed a shorter panicle length, fewer spikelets per panicle and a lower plant grain yield, the plant also exhibited a lower plant height. Taken together, our results revealed that theqph12have good specific application prospects in future rice breeding.
Source: Frontiers in Plant Science
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W4281902152