The complete mitochondrial genome of Parasitus fimetorum (Berlese, 1904) (Arachnida: Parasitiformes: Parasitidae)
Year: 2022
Type: article
Abstract: Background The family Parasitidae (Arachnida: Acari) includes 426 species. Up to now, the complete mitochondrial genomes of only one Parasitidae species (Parasitus wangdunqingi) has been sequenced, and it was discovered that the mitochondrial genome of this species have retained the ancestral mitochondrial genome organization of arthropods. The scarcity of available sequence data has greatly impeded evolutionary studies in Acari (mites and ticks). Here, we report the complete mitochondrial genome of Parasitus fimetorum (Family Parasitidae) and provide a comparison to Parasitus wangdunqingi. Methods We use a PCR-based strategy to amplify the mitochondrial genome of Parasitus fimetorum, and these amplicons are sequenced using the Illumina Hiseq X-10 platform. Phylogeny of Parasitiformes mites based on cox1 gene of 51 species using maximum likelihood (ML) methods. Result The complete mt genome of P. fimetorum is a typical circular, double-stranded DNA molecule 14,619 bp in size that contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. The mitochondrial gene order of P. fimetorum was similar to that of the arthropods hypothesized ancestor. Mitochondrial genomes of P. fimetorum and P. wangdunqingi were compared and analyzed with the following characteristics: (1) both parasitus species evolved independently rather than synergically; (2) the preference of the two mites for codons is different, which may be due to the mutation pressure of certain mites. Phylogenetic analyses based on sequences of the cox1 genes showed that Parasitidae was monophyletic. Conclusion The mitochondrial genomes of Parasitidae mites likely remained the ancestral mt genome organization of arthropods and phylogeny of Parasitiformes mites based on cox1 gene sequences indicates that different lineages are basically monophyletic, and strong supports the current morphological taxonomy. Among others, this new resource will provided thoroughly and systematically information for further study on the mitogenomes, phylogenetics and taxonomic revision of the mites.
Source: Mitochondrial DNA Part B
Authors: Huijuan Yang, Ting Chen, Wenge Dong
Institution: Dali University
Language: English
Topic: Study of Mite Species
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4282964513