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Whole Mount In Situ Hybridization in Zebrafish v1

Year: 2022

Type: preprint

Abstract: Whole mount in situ hybridization protocol optimized for single gene detection using chromogenic substrates NBT/BCIP in zebrafish (Danio rerio). Options are included for bleaching and permeabilization. This protocol beings with tissue preparation and ends with glycerol mounting for imaging. Probe synthesis is not included. This protocol has been used for embryos/larvae from 24 - 72 hpf.

DOI: 10.17504/protocols.io.b74vrqw6

Author: D R Hammond-Weinberger

Language: English

Topic: Zebrafish Biomedical Research Applications

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4283372901

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