Work

Identification of Antimycobacterial from Actinobacteria (INACC A758) Secondary Metabolites using Metabolomics Data

Year: 2022

Type: article

Abstract: Actinobacteria produce active secondary metabolite with medicinal properties, such as antibacterial or anticancer. However, there are some reports about the difficulties in discovering novel secondary metabolites. Therefore, the need for a new approach is obvious. Several factors such as types of nutrients in the culture media or different solvents used for extraction have been proven to influence the Actinobacteria secondary metabolite production. In this study, a combination of culture media optimization and metabolites fingerprint analysis were applied to identify antimycobacterial active compounds from Actinobacteria (InaCC A758). Five culture media were used in the secondary metabolite production of the Actinobacteria. The metabolite fingerprinting was carried out by analyzing the secondary metabolite profile extracted from culture media optimization using UPLC-MS. Multivariate analysis, i.e. cluster analysis and principal component analysis (PCA) was applied. The result showed that a unique antimycobacterial compound candidate against Mycobacterium smegmatis was produced by SYP media cultured InaCC A758 (MIC 6.25 µg/mL).

Source: Sains Malaysiana

DOI: 10.17576/jsm-2022-5105-16

Authors: Maya Dian Rakhmawatie Maya Dian Rakhmawatie, Mustofa Mustofa, Puspita Lisdiyanti Puspita Lisdiyanti, Woro Rukmi Pratiwi Woro Rukmi Pratiwi, Tri Wibawa Tri Wibawa

Institutions: Indonesian Institute of Sciences, Universitas Gadjah Mada

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Applied Microbiology and Biotechnology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Kementerian Riset, Teknologi dan Pendidikan Tinggi