Work

Covalently attached intercalators restore duplex stability and splice-switching activity to triazole-modified oligonucleotides

Year: 2022

Type: article

Abstract: distribution. Triazole linkages, however, are known to reduce RNA target binding affinity. Here we show that by attaching pyrene or anthraquinone to the ribose sugar on the 5'-side of the triazole, it is possible to recover duplex stability and restore the splice switching ability of triazole-containing oligonucleotides.

Source: RSC Chemical Biology

DOI: 10.1039/d2cb00100d

Authors: Anna Dysko, Ysobel R. Baker, Graham McClorey, Matthew J. A. Wood, Sabine Fenner, Glynn Williams, Afaf El-Sagheer, Tom Brown

Institutions: University of Oxford, Suez University

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,962

Funders: University of Oxford, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council

Awards: G2068366838, Advancing Oligonucleotide Therapeutics, High-sensitivity multinuclear 600 MHz NMR for synthesis, catalysis and functional materials, New oligonucleotide analogues for therapeutic applications, New and versatile chemical approaches for the synthesis of mRNA and tRNA

OpenAlex ID: W4283662630

FWCI: 0.365

Cites: 48

Cited by: 6

Related to: 10