In vitro assessment of apoptosis induced by phytochemicals from Ampelocissus latifolia (Roxb.) Planch
Year: 2020
Type: article
Abstract: Department of Zoology, Lady Brabourne College, Suhrawardy Avenue, Beniapukur, Kolkata-700 017, India Department of Zoology, Durgapur Govt. College, Jawahar Lal Nehru Road, Amarabati Colony, Durgapur-713 214, West Bengal, India Department of Chemistry, Sidho-Kanho-Birsha University, Purulia-723 104, West Bengal, India Molecular Biology and Genetics Unit, Department of Zoology, The University of Burdwan, Golapbag, Burdwan-713 104, West Bengal, India E-mail: [email protected] Manuscript received online 03 December 2020, accepted 28 December 2020 This study evaluated the apoptotic and cytotoxic activities of aerial parts’ hydro-methanolic extract of Ampelocissus latifolia (Roxb.) Planch. (AHMEAL) on in vitro cultured Dalton’s lymphoma (DL) cells. Here, in vitro cultured DL cells were exposed to AHMEAL for 24 h. Cell viability assays like trypan blue assay, succinate dehydrogenase activity assay (MTT assay), live/dead assay through fluorescence microscopy, and flow cytometric analysis were done for the study of in vitro cytotoxic activity. Results indicated a concentration-dependent decreased survivability, increased percentages of apoptotic cells along with increasing sub-G1 cell populations in treated DL cells. Steroids, carbohydrates, flavonoids, terpenoids, alkaloids, tannins, glycosides, anthraquinones, and saponins are also present in AHMEAL. Thus, the outcomes of this study reveal that the phytochemicals from AHMEAL hold a significant apoptotic potential and can be targeted for the invention of chemotherapeutic drugs.
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4287571176