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Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase from Penicillium oxalicum sp. 68

Year: 2022

Type: article

Abstract: had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.2204.04012

Authors: Tong Zhou, Yanbo Hu, Xuecui Yan, Jing Cui, Yibing Wang, Feng Luo, Ye Yuan, Zhenxiang Yu, Yifa Zhou

Institutions: First Hospital of Jilin University, Changchun University, Northeast Normal University, Changchun Normal University

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W4287842888

FWCI: 0.452

Cites: 46

Cited by: 5

Related to: 10