Quenching of virulence factors and expression of LasI and RhlR genes of quorum sensing by natural compounds in Pseudomonas aeruginosa isolates
Year: 2022
Type: article
Abstract: Salicylic acid (SA) and cinnamaldehyde (CA) have the ability to kill microorganisms, but at certain concentrations, they can reduce virulence factors without affecting bacterial growth. This study aimed to know activity of SA and CA in its sub-inhibitory levels on virulence factors and on expiration of QS genes LasI and RhlR. Minimum inhibitory concentration (MIC) of CA and SA were determined. The sub-MIC of SA and CA were utilized to examine their inhibitory effect on biofilm formation, Pyocyanin, and gene expression levels of LasI and RhlR. the result showed both compounds had an inhibitory effect on biofilm formation, Pyocyanin with significant differences. The mean (±SD) of Biofilm formation was decreased from 0.208 (±0.156) to 0.119 (±0.097) with SA and to 0.098 (±0.079) with CA, while the pyocyanin was reduced from 4.76 (±2.96) to 4.28 (±2.27) with SA and to 1.59 (±1.21) with CA. the expression levels of LasI and RhlR decreased; the mean of RhlR cycle threshold (CT) were increased from 19.8 to 21.5, and 22.28. while (CT) of LasI was increased from 20.7 to 21.16and 21.8 with SA, CA respectively. These impressive results breathe hope in the fight against resistant P. aeruginosa by suppressing its QS-regulated virulence factors.
Institution: University of Anbar
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4288070537