Work

Experimental evolution of Vibrio cholerae identifies hypervesiculation as a way to increase motility in the presence of polymyxin B

Year: 2022

Type: article

Abstract: Vibrio cholerae is the bacteria responsible for the cholera disease and a natural inhabitant of aquatic environments. Biofilm formation is important for human colonization and environmental survival. Motility is essential for adhesion and biofilm formation by V. cholerae . In a previous study, we showed that motility and biofilm formation are altered in the presence of sub-inhibitory concentrations of polymyxin B in V. cholerae . In this study, we performed an experimental evolution to identify genes rescuing the motility in the presence of polymyxin B. Mutations in 5 genes have been identified: ihfA, vacJ ( mlaA ), mlaF, dacB and ccmH . The details of these mutations, their potential impact on the function of the proteins they encode and on the motility in presence of polymyxin B are discussed.

Source: Frontiers in Microbiology

DOI: 10.3389/fmicb.2022.932165

Authors: Sean Giacomucci, Annabelle Mathieu-Denoncourt, Antony T. Vincent, Hanen Jannadi, Marylise Duperthuy

Institutions: Université de Montréal, Université Laval

Language: English

Topic: Vibrio bacteria research studies

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,950