Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside
Year: 2022
Type: article
Abstract: Microtubules play important roles in biological functions by forming superstructures, such as doublets and branched structures, in vivo. Despite the importance, it is challenging to construct these superstructures in vitro. Here, we designed a tetrameric fluorescent protein Azami-Green (AG) fused with His-tag and Tau-derived peptide (TP), TP-AG, to generate the superstructures. Main binding sites of TP-AG can be controlled to the inside and outside of microtubules by changing the polymerization conditions. The binding of TP-AG to the inside promoted microtubule formation and generated rigid and stable microtubules. The binding of TP-AG to the outside induced various microtubule superstructures, including doublets, multiplets, branched structures, and extremely long microtubules by recruiting tubulins to microtubules. Motile microtubule aster structures were also constructed by TP-AG. The generation of various microtubule superstructures by a single type of exogenous protein is a new concept for understanding the functions of microtubules and constructing microtubule-based nanomaterials.
Source: Science Advances
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $4,500
Awards: Measurement of protein translocation by SecA ATPase, G1948646822, G2023316581, Molecular swarm robotics: Verifying working ability of molecular robots and their implementation in social contexts, 内部構造操作による微小管の機能進化, G4473254024, Integrated Structural Analysis of the Ciliary Central Pair Complex, G513247972, 繊毛の運動機構の原子レベルでの解明, タンパク質内包を基盤とした微小管の光制御による細胞操作, G6017436553, Generation of atomic model of more intact doublet microtubule structure, Structural rigidification of microtubules by peptide-based encapsulation of nanostructures, G8158591127, ホロトランスロコン複合体による効率的タンパク質分泌の構造基盤, Fabrication of artificial muscles from biomolecular motors and DNA origami nanostructures
OpenAlex ID: W4294884844